Parsippany, NJ – 25 June 2025 – A new preclinical study, conducted by Pudenz et al.,[i] demonstrates that combining chicory-derived inulin-type fructans with the human milk oligosaccharide (HMO) 2’-fucosyllactose (2’-FL) leads to additive, and even synergistic, effects on toddler gut microbiota. As one of the first studies to test combinations of prebiotics from chicory root with 2’-FL, it strengthens the argument for prebiotic blends to be used in milk formulas to support early-life gut health, with potential long-term benefits for digestive and immune function.
The science-driven, ingredient-specific research, conducted in collaboration with leading academic partner ETH Zurich, focused on microbial composition and metabolic effects in early nutrition. As part of the in vitro fermentation study, stool samples from toddlers were tested using PolyFermS® long-term continuous fermentation model – a sophisticated technology that preserves the individual microbial community and biodiversity of each donor. It also mimics the dynamics of the large intestine, allowing donor-specific and reproducible insights into gut fermentation.
The study abstract was first published as an e-poster during the 57th Annual Meeting of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition in Finland – a major event in the pediatric nutrition field in Europe.
The findings showed that inulin-type fructans induced metabolic and compositional changes in the gut microbiota, while 2’-FL alone had less prominent effects. However, the combination of fructans and 2’-FL led to additive or synergistic effects and enhanced the production of the beneficial metabolite butyrate, an important fuel for gut cells. This correlated with an increase in beneficial bacteria, including Faecalibacterium and other members of the Lachnospiraceae family. In addition, the novel prebiotic combination of 2’-FL and inulin-type fructans promoted the bifidogenic effect, i.e., the growth of bifidobacteria, depending on the initial microbiota. While this growth was observed in all samples, the type of Bifidobacterium species that increased, varied by donor.
The results demonstrate that the combination of prebiotics from chicory root and 2’-FL can more effectively support the development of a healthy gut microbiota in toddlers than the two ingredients individually. This finding further supports the role of scientifically-backed prebiotic blends in complementing early-life nutrition, especially for toddlers transitioning from breastfeeding. The varying findings across the samples highlight individual differences and the potential for personalized nutrition approaches.
Stephan Theis, Head of Nutrition Science and Communication at BENEO, commented: “These new insights underline the power of combining prebiotics from chicory root with the human milk oligosaccharide, 2’-FL, to more closely replicate the natural gut-supporting effects of breastmilk – a promising direction for smart solutions in toddler nutrition.”
While 2’-FL is the most abundant and extensively studied HMO, prebiotics from chicory root belong to the very few proven prebiotics according to ISAPP[ii], and are the only plant-based ones available. The new study reinforces BENEO’s leading role in researching prebiotics from chicory root, leveraging extensive expertise to enhance baby food with functional ingredients. Thanks to its strategic global partnership with WACKER, BENEO is offering the HMO 2’-FL, reaffirming its commitment to bridging the gap between formula and the natural composition of breast milk.
ENDS
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The BENEO-Institute is an organization which brings together BENEO’s expertise from Nutrition Science and Regulatory teams. It acts as an advisory body for customers and partners reaching from ingredient approval, physiological effects and nutritional composition to communication, education, and labelling. The BENEO-Institute works on topics related to the microbiome and prebiotics, digestive health and well-being, blood sugar management and the achievement of lower glycaemic diets, cognition and mental health, as well as bone health. It also addresses nutrition related questions regarding protein and fibre intake requirements, sugar reduction and more.
The BENEO-Institute facilitates access to the latest scientific research and knowledge throughout all nutritional and regulatory topics related to BENEO ingredients. It provides BENEO customers and partners with substantiated guidance for some of the most critical questions in the food and feed industry, as well as public health. BENEO, a division of the Südzucker Group, employs more than 1,200 people, and has production units in Belgium, Chile, Germany, Italy and the Netherlands.
[i] Pudenz M, Theis S, Bircher L, van Harsselaar J, Lacroix C (2025) Identification of Synergistic Effects of Fructan and HMO Combinations on Toddler Gut Microbiota Using an In Vitro Model of Intestinal Fermentation (POLYFERMS®). ESPGHAN 57th Annual Meeting Abstracts. JPGN Reports 6(S1):S1610. https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpr3.70024
[ii] Gibson GR, Hutkins R, Sanders ME et al. (2017) Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol 14(8): 491–502. https://www.nature.com/articles/nrgastro.2017.75.pdf See also: https://isappscience.org/